Use of Chitosan Conduit for Bridging Small-Gap Peripheral Nerve Defect in Sciatic Nerve Transection Model of Rat

Objective-To evaluate effect of chitosan conduit for peripheral nerve regeneration using sciatic nerve transection model in rat DesignExperimental in vivo study. AnimalsSixty healthy male Wistar rats. Procedures-The rats were divided into four experimental groups (n=15) randomly. In sham group the left sciatic nerve was exposed through a gluteal muscle incision and after careful homeostasis the wound was sutured. In transected control group the left sciatic nerve was exposed the same way, transected proximal to the tibio-peroneal bifurcation leaving a 10 mm gap and the nerve ends were sutured to the adjacent muscles. In silicone or chitosan groups the left sciatic nerve was transected the same way and proximal and distal stumps were each inserted into a silicone or chitosan tube. Each group was further subdivided into three subgroups of five animals each and were studied 4, 8, 12 weeks post operatively. ResultsFunctional and electrophysiological analyses showed significant improvement of nerve function in chitosan than in silicone group (P < 0.05). Morphometric indices and immuohistochemistry indicated that there were significant differences (P < 0.05) between chitosan and silicone with transected control groups 12 weeks after surgery. ∗ Corresponding author: Saeed Azizi, DVM, DVSc, Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran. E-mail: [email protected]